Validation Data Gallery
Tested Applications
| Positive WB detected in | NIH3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
35002-1-AP targets Cyclophilin B-specific in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Protein 相同性解析による交差性が予測される生物種 |
| Full Name | peptidylprolyl isomerase B (cyclophilin B) |
| Calculated molecular weight | 24 kDa |
| Observed molecular weight | 24 kDa |
| GenBank accession number | NM_000942 |
| Gene Symbol | Cyclophilin B |
| Gene ID (NCBI) | 5479 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P23284 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
PPIB, also named as CYPB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. This antibody is specific to PPIB.