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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cyclophilin A Polyclonal antibody

Cyclophilin A Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 51040-1-AP

Synonyms

Cyclophilin A, Cyclosporin A binding protein, CYPA, CYPH, PPIA, PPIase A, Rotamase A

Validation Data Gallery

Applications:
  • WB analysis of Jurkat using 51040-1-AP

    WB analysis of Jurkat using 51040-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 51040-1-AP (Cyclophilin A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy PPIA Ready-To-Use IHC Kit. PPIA primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

51040-1-AP targets Cyclophilin A in WB,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cyclophilin A fusion protein Ag0480 相同性解析による交差性が予測される生物種
Full Name peptidylprolyl isomerase A (cyclophilin A)
Calculated molecular weight 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC000689
Gene symbol PPIA
Gene ID (NCBI) 5478
RRIDAB_2237515
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

PPIA, also named as CYPA and Rotamase A, belongs to the cyclophilin-type PPIase family and PPIase A subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIA forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. PPIA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication.

Protocols

Product Specific Protocols
WB protocol for Cyclophilin A antibody 51040-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Oncol

Proteomics-based identification of a group of apoptosis-related proteins and biomarkers in gastric cancer.

Authors - Zhigang Bai
View Article