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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPFIA4 Polyclonal antibody

PPFIA4 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 25295-1-AP

Synonyms

KIAA0897, Liprin alpha 4, PPFIA4

Validation Data Gallery

Applications:
  • IHC staining of mouse brain using 25295-1-AP

    IHC staining of mouse brain using 25295-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25295-1-AP (PPFIA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 25295-1-AP

    IHC staining of mouse brain using 25295-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25295-1-AP (PPFIA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 25295-1-AP

    IHC staining of rat brain using 25295-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25295-1-AP (PPFIA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 25295-1-AP

    IHC staining of rat brain using 25295-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25295-1-AP (PPFIA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

25295-1-AP targets PPFIA4 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPFIA4 fusion protein Ag18130 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 4
Calculated molecular weight 701 aa, 78 kDa
GenBank accession numberBC132925
Gene symbol PPFIA4
Gene ID (NCBI) 8497
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tyrosine phosphatase receptor type F polypeptide interacting protein alpha 4 (PPFIA4), also named as Liprin alpha 4, belongs to the liprin family and Liprin-alpha subfamily. PPFIA4 has been reported as a new potential therapeutic target for refractory pancreatic cancer, small cell lung cancer, and clear cell renal cell cancer (PMID: 35382861). As a glycolysis-related oncogene, PPFIA4 is also a potential biomarker of colon cancer (PMID: 34094943).

Protocols

Product Specific Protocols
IHC protocol for PPFIA4 antibody 25295-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols