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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPFIA3 Polyclonal antibody (11492-1-AP)

    WB analysis using 11492-1-AP (same clone as 11492-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11492-1-AP (PPFIA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11492-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using PPFIA3 Polyclonal antibody (11492-1-AP)

    WB analysis of mouse brain using 11492-1-AP (same clone as 11492-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11492-1-AP (PPFIA3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11492-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PPFIA3 Polyclonal antibody (11492-1-AP)

    WB analysis of HEK-293 using 11492-1-AP (same clone as 11492-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11492-1-AP (PPFIA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11492-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PPFIA3 Polyclonal antibody (11492-1-AP)

    IP experiment of mouse brain using 11492-1-AP (same clone as 11492-1-PBS)

    IP result of anti-PPFIA3 (IP:11492-1-AP, 4ug; Detection:11492-1-AP 1:600) with mouse brain tissue lysate 3440ug. This data was developed using the same antibody clone with 11492-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using PPFIA3 Polyclonal antibody (11492-1-AP)

    IHC staining of mouse cerebellum using 11492-1-AP (same clone as 11492-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11492-1-AP (PPFIA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11492-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using PPFIA3 Polyclonal antibody (11492-1-AP)

    IHC staining of mouse cerebellum using 11492-1-AP (same clone as 11492-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11492-1-AP (PPFIA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11492-1-PBS in a different storage buffer formulation.

PPFIA3 Polyclonal antibody, PBS Only

PPFIA3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 11492-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

PTPRF-interacting protein alpha-3, Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-3, Liprin-alpha-3, KIAA0654

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11492-1-PBS targets PPFIA3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1971

Product name: Recombinant human PPFIA3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-277 aa of BC021255

Sequence: APASSRTSTGNVWMTHEEMESLTATTKPILAYGDMNHEWVGNDWLPSLGLPQYRSYFMESLVDARMLDHLNKKELRGQLKMVDSFHRVSLHYGIMCLKRLNYDRKDLERRREESQTQIRDVMVWSNERVMGWVSGLGLKEFATNLTESGVHGALLALDETFDYSDLALLLQIPTQNAQARQLLEKEFSNLISLGTDRRLDEDSAKSFSRSPSWRKMFREKDLRGVTPDSAEMLPPNFRSAAAGALGSPGLPLRKLQPEGQTSGSSRADGVSVRTYSC

相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3
Calculated molecular weight 1194 aa, 133 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC021255
Gene Symbol PPFIA3
Gene ID (NCBI) 8541
RRIDAB_2171628
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75145
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PPFIA3, also named as KIAA0654, belongs to the liprin family and Liprin-alpha subfamily. PPFIA3 may regulate the disassembly of focal adhesions. It may localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates. PPFIA3 exhibites more than 5 fold accumulations in the monoribosome fraction. PPFIA3 is decreased at the protein level by the miR-99 family but not in the luciferase reporter with the 3'UTR of PPFIA3. (PMID:21212412)

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