Validation Data Gallery
Tested Applications
Positive WB detected in | mouse kidney tissue, rat kidney tissue |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
31543-1-AP targets PPEF2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PPEF2 fusion protein Ag35922 相同性解析による交差性が予測される生物種 |
Full Name | protein phosphatase, EF-hand calcium binding domain 2 |
Calculated molecular weight | 86kDa |
Observed molecular weight | 69 kDa |
GenBank accession number | NM_006239 |
Gene Symbol | PPEF2 |
Gene ID (NCBI) | 5470 |
RRID | AB_3670032 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity Purification |
UNIPROT ID | O14830 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Pprotein phosphatase with EF-hand domain 2 (PPEF2), also known as PPP7CB, is a member of the serine/threonine protein phosphatase with EF-hand motif family. PPEF2 can dephosphorylate ubiquitin and suppress PINK1-dependent mitophagy. PPEF2 functions to suppress mitochondrial quality control on a cellular level through dephosphorylation of p-S65 ubiquitin. PPEF2 exists 2 isoforms produced by alternative splicing with a molecular weight of 86 kDa (PPEF-2(L)) and 69 kDa (PPEF-2(S)).
Protocols
Product Specific Protocols | |
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WB protocol for PPEF2 antibody 31543-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |