Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse kidney tissue, rat kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31543-1-AP targets PPEF2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PPEF2 fusion protein Ag35922 相同性解析による交差性が予測される生物種 |
| Full Name | protein phosphatase, EF-hand calcium binding domain 2 |
| Calculated molecular weight | 86kDa |
| Observed molecular weight | 69 kDa |
| GenBank accession number | NM_006239 |
| Gene Symbol | PPEF2 |
| Gene ID (NCBI) | 5470 |
| RRID | AB_3670032 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O14830 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Pprotein phosphatase with EF-hand domain 2 (PPEF2), also known as PPP7CB, is a member of the serine/threonine protein phosphatase with EF-hand motif family. PPEF2 can dephosphorylate ubiquitin and suppress PINK1-dependent mitophagy. PPEF2 functions to suppress mitochondrial quality control on a cellular level through dephosphorylation of p-S65 ubiquitin. PPEF2 exists 2 isoforms produced by alternative splicing with a molecular weight of 86 kDa (PPEF-2(L)) and 69 kDa (PPEF-2(S)).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PPEF2 antibody 31543-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |