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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPDPF Polyclonal antibody (19912-1-AP)

    WB analysis using 19912-1-AP (same clone as 19912-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19912-1-AP (PPDPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19912-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse pancreas tissue using PPDPF Polyclonal antibody (19912-1-AP)

    WB analysis of mouse pancreas using 19912-1-AP (same clone as 19912-1-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19912-1-AP (PPDPF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19912-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PPDPF Polyclonal antibody (19912-1-AP)

    IHC staining of human colon cancer using 19912-1-AP (same clone as 19912-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19912-1-AP (C20orf149 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19912-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using PPDPF Polyclonal antibody (19912-1-AP)

    IHC staining of human pancreas using 19912-1-AP (same clone as 19912-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 19912-1-AP (PPDPF antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19912-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using PPDPF Polyclonal antibody (19912-1-AP)

    IHC staining of human pancreas using 19912-1-AP (same clone as 19912-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 19912-1-AP (PPDPF antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19912-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PPDPF Polyclonal antibody (19912-1-AP)

    IF Staining of U2OS using 19912-1-AP (same clone as 19912-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PPDPF antibody (19912-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 19912-1-PBS in a different storage buffer formulation.

PPDPF Polyclonal antibody, PBS Only

PPDPF Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 19912-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

C20orf149, EXPDF

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19912-1-PBS targets PPDPF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13740

Product name: Recombinant human C20orf149 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-114 aa of BC002531

Sequence: MAAIPSSGSLVATHDYYRRRLGSTSSNSSCSSTECPGEAIPHPPGLPKADPGHWWASFFFGKSTLPFMATVLESAEHSEPPQASSSMTACGLARDAPRKQPGGQSSTASAGPPS

相同性解析による交差性が予測される生物種
Full Name chromosome 20 open reading frame 149
Calculated molecular weight 114 aa, 12 kDa
Observed molecular weight 10-12 kDa
GenBank accession numberBC002531
Gene Symbol PPDPF
Gene ID (NCBI) 79144
RRIDAB_10642438
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H3Y8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Pancreatic progenitor cell differentiation and proliferation factor (PPDPF), also known as C20orf149 and EXPDF, belongs to the PPDPF family. PPDPF is a key regulator for the development of exocrine pancreas. PPDPF is highly expressed in various cancers, including HCC, colorectal cancer, prostate cancer, etc (PMID: 34031390, 34975328, 37027301). PPDPF consists of 114 amino acids and the molecular weight is 12 kDa.

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