Validation Data Gallery

Filter: AllWBIHC

WB analysis of HeLa using 18001-1-AP (same clone as 18001-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 18001-1-AP (PPCS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.
WB analysis using 18001-1-AP (same clone as 18001-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 18001-1-AP (PPCS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 18001-1-AP (same clone as 18001-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 18001-1-AP (same clone as 18001-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 18001-1-AP (same clone as 18001-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 18001-1-AP (same clone as 18001-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 18001-1-AP (same clone as 18001-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 18001-1-AP (same clone as 18001-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 18001-1-AP (same clone as 18001-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 18001-1-AP (same clone as 18001-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 18001-1-AP (same clone as 18001-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 18001-1-AP (same clone as 18001-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 18001-1-AP (same clone as 18001-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 18001-1-AP (same clone as 18001-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.

PPCS Polyclonal antibody, PBS Only

PPCS Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 18001-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

COAB, EC:6.3.2.51, Phosphopantothenate--cysteine ligase, PPC synthetase

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18001-1-PBS targets PPCS in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12491 Product name: Recombinant human PPCS protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-311 aa of BC106064 Sequence: MAEMDPVAEFPQPPGAARWAEVMARFAARLGAQGRRVVLVTSGGTKVPLEARPVRFLDNFSSGRRGATSAEAFLAAGYGVLFLYRARSAFPYAHRFPPQTWLSALRPSGPALSGLLSLEAEENALPGFAEALRSYQEAAAAGTFLAVEFTTLADYLHLLQAAAQALNPLGPSAMFYLAAAVSDFYVPVSEMPEHKIQSSGGPLQITMKMVPKLLSPLVKDWAPKAFIISFKLETDPAIVINRARKALEIYQHQVVVANILESRQSFVFIVTKDSETKLLLSEEEIEKGVEIEEKIVDNLQSRHTAFIGDRN 相同性解析による交差性が予測される生物種
Full Name	phosphopantothenoylcysteine synthetase
Calculated molecular weight	311 aa, 34 kDa
Observed molecular weight	34 kDa
GenBank accession number	BC106064
Gene Symbol	PPCS
Gene ID (NCBI)	79717
RRID	AB_10548725
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9HAB8
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

PPCS(Phosphopantothenate--cysteine ligase) is also named as COAB and belongs to the PPC synthetase family. It catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. It can be exsit as a homodimer(PMID:12906824).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 18001-1-AP (same clone as 18001-1-PBS)

WB analysis using 18001-1-AP (same clone as 18001-1-PBS)

IHC staining of human kidney using 18001-1-AP (same clone as 18001-1-PBS)

IHC staining of human kidney using 18001-1-AP (same clone as 18001-1-PBS)

IHC staining of human placenta using 18001-1-AP (same clone as 18001-1-PBS)

IHC staining of human placenta using 18001-1-AP (same clone as 18001-1-PBS)

IHC staining of human testis using 18001-1-AP (same clone as 18001-1-PBS)

IHC staining of human testis using 18001-1-AP (same clone as 18001-1-PBS)

IHC staining of human spleen using 18001-1-AP (same clone as 18001-1-PBS)