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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPCDC Polyclonal antibody

PPCDC Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16270-1-AP

Synonyms

CoaC, MDS018, PPC DC, PPCDC, UNQ9365/PRO34154

Validation Data Gallery

Applications:
  • WB analysis of human placenta using 16270-1-AP

    WB analysis of human placenta using 16270-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 16270-1-AP (PPCDC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 16270-1-AP

    WB analysis using 16270-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16270-1-AP (PPCDC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells, human placenta tissue, HL-60 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

16270-1-AP targets PPCDC in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPCDC fusion protein Ag9337 相同性解析による交差性が予測される生物種
Full Name phosphopantothenoylcysteine decarboxylase
Calculated molecular weight 204 aa, 22 kDa
Observed molecular weight22-25 kDa, 14 kDa
GenBank accession numberBC014409
Gene symbol PPCDC
Gene ID (NCBI) 60490
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCDC, one of the last enzymes in this pathway, converts phosphopantothenoylcysteine to 4-prime-phosphopantetheine. The antibody was generated against full length human recombinant PPCDC, which is a bright yellow protein, indicating the presence of flavins. The antibody solution gives yellow color, too. It may be caused by elution of flavins during affinity purification with PPCDC-protein column.

Protocols

Product Specific Protocols
WB protocol for PPCDC antibody 16270-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols