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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using CXCL7/PPBP Polyclonal antibody (13313-1-AP)

    WB analysis of mouse liver using 13313-1-AP (same clone as 13313-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 13313-1-AP (PPBP,NAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13313-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human plasma using CXCL7/PPBP Polyclonal antibody (13313-1-AP)

    WB analysis of human plasma using 13313-1-AP (same clone as 13313-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 13313-1-AP (PPBP,NAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13313-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using CXCL7/PPBP Polyclonal antibody (13313-1-AP)

    WB analysis of human liver using 13313-1-AP (same clone as 13313-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 13313-1-AP (PPBP,NAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13313-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using CXCL7/PPBP Polyclonal antibody (13313-1-AP)

    IHC staining of mouse placenta using 13313-1-AP (same clone as 13313-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 13313-1-AP (CXCL7/PPBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13313-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CXCL7/PPBP Polyclonal antibody (13313-1-AP)

    IHC staining of human placenta using 13313-1-AP (same clone as 13313-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13313-1-AP (CXCL7/PPBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13313-1-PBS in a different storage buffer formulation.

CXCL7/PPBP Polyclonal antibody, PBS Only

CXCL7/PPBP Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 13313-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

PPBP,NAP2, CXCL7, NAP-2, PPBP, PPBP/NAP2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13313-1-PBS targets CXCL7/PPBP in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4147

Product name: Recombinant human PPBP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 31-128 aa of BC028217

Sequence: TALASSTKGQTKRNLAKGKEESLDSDLYAELRCMCIKTTSGIHPKNIQSLEVIGKGTHCNQVEVIATLKDGRKICLDPDAPRIKKIVQKKLAGDESAD

相同性解析による交差性が予測される生物種
Full Name pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)
Calculated molecular weight 128 aa, 14 kDa
Observed molecular weight 8-14 kDa
GenBank accession numberBC028217
Gene Symbol PPBP
Gene ID (NCBI) 5473
RRIDAB_10597238
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP02775
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PPBP, also named as CXCL7, NAP2, or β-Thromboglobulin, is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.

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