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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPA2 Polyclonal antibody

PPA2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16662-1-AP

Synonyms

HSPC124, PPA2, PPase 2, pyrophosphatase (inorganic) 2, Pyrophosphatase SID6 306, SID6 306

Validation Data Gallery

Applications:
  • WB analysis of mouse heart using 16662-1-AP

    WB analysis of mouse heart using 16662-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16662-1-AP (PPA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 16662-1-AP

    WB analysis using 16662-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16662-1-AP (PPA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse kidney using 16662-1-AP

    WB analysis of mouse kidney using 16662-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16662-1-AP (PPA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 16662-1-AP

    IHC staining of human liver cancer using 16662-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPA2 antibody included
  • IHC staining of human liver cancer using 16662-1-AP

    IHC staining of human liver cancer using 16662-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPA2 antibody included
  • IHC staining of human liver cancer using 16662-1-AP

    IHC staining of human liver cancer using 16662-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPA2 antibody included
  • IHC staining of human liver cancer using 16662-1-AP

    IHC staining of human liver cancer using 16662-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPA2 antibody included
  • IHC staining of human lung cancer using 16662-1-AP

    IHC staining of human lung cancer using 16662-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPA2 antibody included
  • IHC staining of human lung cancer using 16662-1-AP

    IHC staining of human lung cancer using 16662-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPA2 antibody included

Tested Applications

Positive WB detected inmouse heart tissue, mouse kidney tissue, mouse liver tissue, rat heart tissue
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PPA2 Ready-To-Use IHC Kit. PPA2 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16662-1-AP targets PPA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPA2 fusion protein Ag10138 相同性解析による交差性が予測される生物種
Full Name pyrophosphatase (inorganic) 2
Calculated molecular weight305aa,35 kDa; 334aa,38 kDa
Observed molecular weight 32-40 kDa
GenBank accession numberBC057219
Gene symbol PPA2
Gene ID (NCBI) 27068
RRIDAB_1851549
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pyrophosphatase 2 (PPA2) is a mitochondrial-located pyrophosphatase (PPase) that hydrolyzes inorganic pyrophosphate (PPi), generated by numerous nucleotide-dependent reactions, into two orthophosphate molecules (PMID: 34400813). PPA2 is a cardiomyopathy-associated protein, which has a greater physiological importance in mitochondrial function (PMID: 27523597).

Protocols

Product Specific Protocols
WB protocol for PPA2 antibody 16662-1-APDownload protocol
IHC protocol for PPA2 antibody 16662-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols