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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPA1 Recombinant antibody (85445-3-RR)

    WB analysis using 85445-3-RR (same clone as 85445-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85445-3-RR (PPA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85445-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using PPA1 Recombinant antibody (85445-3-RR)

    IHC staining of human ovary cancer using 85445-3-RR (same clone as 85445-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85445-3-RR (PPA1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85445-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using PPA1 Recombinant antibody (85445-3-RR)

    IHC staining of human ovary cancer using 85445-3-RR (same clone as 85445-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85445-3-RR (PPA1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85445-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85445-3-RR

    Affinity and Kinetic Characterization of 85445-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85445-3-RR against Human PPA1 were performed. The affinity constant is 4.07 nM.

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PPA1 Recombinant antibody, PBS Only

PPA1 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85445-3-PBS
Clone No.242963D1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

242963D1, EC:3.6.1.1, Inorganic pyrophosphatase, IOPPP, PP

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85445-3-PBS targets PPA1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PPA1 fusion protein Ag6969 相同性解析による交差性が予測される生物種
Full Name pyrophosphatase (inorganic) 1
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC001022
Gene Symbol PPA1
Gene ID (NCBI) 5464
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15181
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PPA1(Inorganic pyrophosphatase) is also named as IOPPP, PP and PPase. It maintains the thermodynamic favorability of these reactions by catalyzing the hydrolysis of pyrophosphates into organic phosphates, which are then exported across the cell membrane(PMID:16300924).The erythrocyte inorganic pyrophosphatase molecule would appear to be a dimer(PMID:4130389).