Validation Data Gallery

Filter: AllWBIHC

WB analysis of SH-SY5Y using 18999-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 18999-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 18999-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 18999-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 18999-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 18999-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 18999-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 18999-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 18999-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 18999-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 18999-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 18999-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human brain using 18999-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 18999-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:100 (under 40x lens).

POU3F3-Specific Polyclonal antibody

POU3F3-Specific Polyclonal Antibody for WB, IHC, ELISA

Cat No. 18999-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

POU3F3, Brain-1, Brain-specific homeobox/POU domain protein 1, BRN1, Oct-8

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	SH-SY5Y cells, HEK-293 cells, human brain tissue, mouse brain tissue
Positive IHC detected in	human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

18999-1-AP targets POU3F3-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	POU class 3 homeobox 3
Calculated molecular weight	50 kDa
Observed molecular weight	66 kDa
GenBank accession number	NM_006236
Gene Symbol	POU3F3
Gene ID (NCBI)	5455
RRID	AB_2284130
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P20264
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

POU3F3, also referred to as OTF8, Brain-1, encodes a member of class III of the POU family of transcription factors, is a well-known transcription factor involved in the development of the central nervous system. POU3F3 is composed by two functional domains needed for the site-specific binding to its target genes: the POU-specific (POU-S) domain and the homeobox domain (POU-H) (PMID: 37165752). Endogenous POU3F3 is upregulated in NSCLC tissues and cells, and high expression of POU3F3 correlated with poor survival prognosis (PMID: 39932442). The antibody is specific to OTF8.

Protocols

Product Specific Protocols
IHC protocol for POU3F3-Specific antibody 18999-1-AP	Download protocol
WB protocol for POU3F3-Specific antibody 18999-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Dev Cell AP-2β/KCTD1 Control Distal Nephron Differentiation and Protect against Renal Fibrosis. Authors - Alexander G Marneros View Article
human	WB	Int Immunopharmacol Iguratimod-encapsulating PLGA-NPs induce human multiple myeloma cell death via reactive oxygen species and Caspase-dependent signalling. Authors - Faming Wang View Article
human	IHC	Histochem Cell Biol Connecting tubules develop from the tip of the ureteric bud in the human kidney. Authors - Beatrix Sarkany View Article
human	WB,IF	Adv Sci (Weinh) Phosphorylation of POU3F3 Mediated Nuclear Translocation Promotes Proliferation in Non-Small Cell Lung Cancer through Accelerating ATP5PF Transcription and ATP Production Authors - Qi-Gang Zeng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of SH-SY5Y using 18999-1-AP

WB analysis of HEK-293 using 18999-1-AP

WB analysis of HEK-293 using 18999-1-AP

WB analysis of human brain using 18999-1-AP

WB analysis of mouse brain using 18999-1-AP

WB analysis of SH-SY5Y using 18999-1-AP

WB analysis of SH-SY5Y using 18999-1-AP

WB analysis of mouse brain using 18999-1-AP

WB analysis of mouse brain using 18999-1-AP

WB analysis of HEK-293 using 18999-1-AP

WB analysis of HEK-293 using 18999-1-AP

WB analysis of SH-SY5Y using 18999-1-AP

IHC staining of human brain using 18999-1-AP

IHC staining of human brain using 18999-1-AP

POU3F3-Specific Polyclonal antibody

POU3F3-Specific Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

AP-2β/KCTD1 Control Distal Nephron Differentiation and Protect against Renal Fibrosis.

Iguratimod-encapsulating PLGA-NPs induce human multiple myeloma cell death via reactive oxygen species and Caspase-dependent signalling.

Connecting tubules develop from the tip of the ureteric bud in the human kidney.

Phosphorylation of POU3F3 Mediated Nuclear Translocation Promotes Proliferation in Non-Small Cell Lung Cancer through Accelerating ATP5PF Transcription and ATP Production