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POU3F3-Specific Polyclonal antibody

POU3F3-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18999-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Brain 1, Brn 1, BRN1, Oct 8, Octamer binding protein 8, OTF 8, OTF8, POU class 3 homeobox 3, POU3F3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inSH-SY5Y cells, HEK-293 cells, human brain tissue, mouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18999-1-AP targets POU3F3-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name POU class 3 homeobox 3
Calculated molecular weight 50 kDa
Observed molecular weight 66 kDa
GenBank accession numberNM_006236
Gene Symbol POU3F3
Gene ID (NCBI) 5455
RRIDAB_2284130
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20264
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OTF8, also named as POU3F3 and BRN1, belongs to the POU transcription factor family and Class-3 subfamily. The antibody is specific to OTF8.

Protocols

Product Specific Protocols
WB protocol for POU3F3-Specific antibody 18999-1-APDownload protocol
IHC protocol for POU3F3-Specific antibody 18999-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Dev Cell

AP-2β/KCTD1 Control Distal Nephron Differentiation and Protect against Renal Fibrosis.

Authors - Alexander G Marneros
humanWB

Int Immunopharmacol

Iguratimod-encapsulating PLGA-NPs induce human multiple myeloma cell death via reactive oxygen species and Caspase-dependent signalling.

Authors - Faming Wang
humanIHC

Histochem Cell Biol

Connecting tubules develop from the tip of the ureteric bud in the human kidney.

Authors - Beatrix Sarkany
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