Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HeLa using 15046-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15046-1-AP (POMP Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15046-1-AP
WB result of POMP antibody (15046-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-POMP transfected HepG2 cells.
WB analysis of HepG2 using 15046-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15046-1-AP (POMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 15046-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 15046-1-AP (POMP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 15046-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15046-1-AP (POMP Antibody) at dilution of 1:50.
IHC staining of human liver using 15046-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15046-1-AP (POMP Antibody) at dilution of 1:50.
IHC staining of human skin using 15046-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 15046-1-AP (POMP Antibody) at dilution of 1:50.
IHC staining of human skin using 15046-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 15046-1-AP (POMP Antibody) at dilution of 1:50.
IF Staining of HepG2 using 15046-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using POMP antibody (15046-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
IF Staining of HeLa using 15046-1-AP
Immunofluorescent analysis of Hela cells, using POMP antibody 15046-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Featured Product
KD/KO Validated

POMP Polyclonal antibody

POMP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15046-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PNAS-110, PNAS 110, hUMP1, HSPC036, HSPC014

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, L02 cells
Positive IHC detected in	human liver tissue, human skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below

Product Information

15046-1-AP targets POMP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6987 Product name: Recombinant human POMP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-141 aa of BC003390 Sequence: MNARGLGSELKDSIPVTELSASGPFESHDLLRKGFSCVKNELLPSHPLELSEKNFQLNQDKMNFSTLRNIQGLFAPLKLQMEFKAVQQVQRLPFLSSSNLSLDVLRGNDETIGFEDILNDPSQSEVMGEPHLMVEYKLGLL 相同性解析による交差性が予測される生物種
Full Name	proteasome maturation protein
Calculated molecular weight	16 kDa
Observed molecular weight	16 kDa
GenBank accession number	BC003390
Gene Symbol	POMP
Gene ID (NCBI)	51371
RRID	AB_10858321
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y244
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for POMP antibody 15046-1-AP	Download protocol
IF protocol for POMP antibody 15046-1-AP	Download protocol
IHC protocol for POMP antibody 15046-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Dermatol Sci The proteasome maturation protein POMP increases proteasome assembly and activity in psoriatic lesional skin. Authors - Barbara A Zieba KD Validated View Article
mouse	WB	JCI Insight Blockade of the CXCR3/CXCL10 axis ameliorates inflammation caused by immunoproteasome dysfunction. Authors - Yuki Sasaki View Article
mouse	WB	Int Immunol Proteasome dysfunction in T cells causes immunodeficiency via cell cycle disruption and apoptosis Authors - Erkhembayar Shinebaatar View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 15046-1-AP

WB analysis of HepG2 using 15046-1-AP

WB analysis of HepG2 using 15046-1-AP

WB analysis of L02 using 15046-1-AP

IHC staining of human liver using 15046-1-AP

IHC staining of human liver using 15046-1-AP

IHC staining of human skin using 15046-1-AP

IHC staining of human skin using 15046-1-AP

IF Staining of HepG2 using 15046-1-AP

IF Staining of HeLa using 15046-1-AP