Validation Data Gallery
Tested Applications
| Positive IHC detected in | human pituitary tissue, mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 ul suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 3 publications below |
| IF | See 15 publications below |
Product Information
66358-1-Ig targets POMC in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17311 Product name: Recombinant human POMC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 27-176 aa of BC065832 Sequence: WCLESSQCQDLTTESNLLECIRACKPDLSAETPMFPGNGDEQPLTENPRKYVMGHFRWDRFGRRNSSSSGSSGAGQKREDVSAGEDCGPLPEGGPEPRSDGAKPGPREGKRSYSMEHFRWGKPVGKKRRPVKVYPNGAEDESAEAFPLEF 相同性解析による交差性が予測される生物種 |
| Full Name | proopiomelanocortin |
| Calculated molecular weight | 267 aa, 29 kDa |
| Observed molecular weight | 35 kDa |
| GenBank accession number | BC065832 |
| Gene Symbol | POMC |
| Gene ID (NCBI) | 5443 |
| RRID | AB_2881737 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P01189 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Pro-opiomelanocortin (POMC) is a prohormone that encodes multiple smaller peptide hormones within its structure. POMC plays important roles in many pathological and physiological processes, including regulating energy metabolism, immune reactions, and sexual functions. POMC expression differs quantitatively and qualitatively among most normal tissues. The large molecule of POMC is the source of several important biologically active substances. POMC can be cleaved enzymatically into the following peptides: d NPP, γ-MSH, ACTH, α-MSH, CLIP, Lipotropin β, Lipotropin γ, β-MSH, β endorphin and Met-enkephalin.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for POMC antibody 66358-1-Ig | Download protocol |
| IF protocol for POMC antibody 66358-1-Ig | Download protocol |
| IHC protocol for POMC antibody 66358-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Phytomedicine Jinkui Shenqi pills ameliorate diabetes by regulating hypothalamic insulin resistance and POMC/AgRP expression and activity | ||
Phytomedicine Jiang Tang San Hao Formula exerts its anti-diabetic effect by affecting the gut-microbiota-brain axis | ||
Arterioscler Thromb Vasc Biol Release of Lipids Stored in the Intestine by Glucagon-Like Peptide-2 Involves a Gut-Brain Neural Pathway | ||
Environ Pollut Phenanthrene exposure disrupts puberty onset in SD rats by influencing hypothalamic neural networks | ||
Front Endocrinol (Lausanne) Involvement of POMC neurons in LEAP2 regulation of food intake and body weight | ||
Int Immunopharmacol Sodium butyrate reduces overnutrition-induced microglial activation and hypothalamic inflammation |