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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POM121 Polyclonal antibody (15645-1-AP)

    WB analysis using 15645-1-AP (same clone as 15645-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15645-1-AP (POM121 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15645-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using POM121 Polyclonal antibody (15645-1-AP)

    IHC staining of human colon using 15645-1-AP (same clone as 15645-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15645-1-AP (POM121 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15645-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using POM121 Polyclonal antibody (15645-1-AP)

    IHC staining of human colon using 15645-1-AP (same clone as 15645-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15645-1-AP (POM121 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15645-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using POM121 Polyclonal antibody (15645-1-AP)

    IF Staining of mouse brain using 15645-1-AP (same clone as 15645-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 15645-1-AP (POM121 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15645-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HCT 116 cells using POM121 Polyclonal antibody (15645-1-AP)

    IF Staining of HCT 116 using 15645-1-AP (same clone as 15645-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using POM121 antibody (15645-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15645-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

POM121 Polyclonal antibody, PBS Only

POM121 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 15645-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

POM121A, NUP121, Nucleoporin Nup121, Nuclear envelope pore membrane protein POM 121A, Nuclear envelope pore membrane protein POM 121

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15645-1-PBS targets POM121 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8177

Product name: Recombinant human POM121 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 635-984 aa of BC008794

Sequence: SQSLHTAVPTATSSSAADFSGFGSTLATSAPATSSQPTLTFSNTSTPTFNIPFGSSAKSPLPSYPGANPQPAFGAAEGQPPGAAKPALAPSFGSSFTFGNSAAPAAAPTPAPPSMIKVVPAYVPTPIHPIFGGATHSAFGLKATASAFGAPASSQPAFGGSTAVFFGAATSSGFGATTQTASSGSSSSVFGSTTPSPFTFGGSAAPAGSGSFGINVATPGSSTTTGAFSFGAGQSGSTATSTPFAGGLGQNALGTTGQSTPFAFNVSSTTESKPVFGGTATPTFGLNTPAPGVGTSGSSLSFGASSAPAQGFVGVAPFGSAALSFSIGAGSKTPGARQRLQARRQHTRKK

相同性解析による交差性が予測される生物種
Full Name POM121 membrane glycoprotein (rat)
Calculated molecular weight 984 aa, 99 kDa
Observed molecular weight 140-150 kDa
GenBank accession numberBC008794
Gene Symbol POM121
Gene ID (NCBI) 9883
RRIDAB_2878160
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96HA1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

POM121 is an integral membrane protein that localizes to the central spoke ring complex and participates in anchoring the nuclear pore complex to the nuclear envelope. Catalog#15645-1-AP do well in identifying the nuclear envelope in immunofluorescence experiments.

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