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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using POLR3C Recombinant antibody (84367-7-RR)

    IF Staining of HeLa using 84367-7-RR (same clone as 84367-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLR3C antibody (84367-7-RR, Clone: 241402D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84367-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84367-7-RR

    Affinity and Kinetic Characterization of 84367-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84367-7-RR against Human POLR3C were performed. The affinity constant is below 1 pM.

POLR3C Recombinant monoclonal antibody, PBS Only

POLR3C Uni-rAb® Recombinant Antibody for IF/ICC, Indirect ELISA
Cat No. 84367-7-PBS
Clone No.241402D3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Indirect ELISA

Synonyms

RPC62, RPC3, RNA polymerase III subunit C3, DNA-directed RNA polymerase III subunit RPC3, DNA-directed RNA polymerase III subunit C

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84367-7-PBS targets POLR3C in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag27311

Product name: Recombinant human POLR3C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 196-397 aa of BC002586

Sequence: IGKGKRRRSSDEDAAGEPKAKRPKYTTDNKEPIPDDGIYWQANLDRFHQHFRDQAIVSAVANRMDQTSSEIVRTMLRMSEITTSSSAPFTQPLSSNEIFRSLPVGYNISKQVLDQYLTLLADDPLEFVGKSGDSGGGMYVINLHKALASLATATLESVVQERFGSRCARIFRLVLQKKHIEQKQVEDFAMIPAKEAKDMLYK

相同性解析による交差性が予測される生物種
Full Name polymerase (RNA) III (DNA directed) polypeptide C (62kD)
Calculated molecular weight 61 kDa
GenBank accession numberBC002586
Gene Symbol POLR3C
Gene ID (NCBI) 10623
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9BUI4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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