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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POLR3C Polyclonal antibody (27864-1-AP)

    WB analysis using 27864-1-AP (same clone as 27864-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27864-1-AP (POLR3C antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27864-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using POLR3C Polyclonal antibody (27864-1-AP)

    IHC staining of mouse kidney using 27864-1-AP (same clone as 27864-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27864-1-AP (POLR3C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27864-1-PBS in a different storage buffer formulation.

POLR3C Polyclonal antibody, PBS Only

POLR3C Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27864-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

DNA-directed RNA polymerase III subunit C, DNA-directed RNA polymerase III subunit RPC3, RNA polymerase III subunit C3, RPC3, RPC62

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27864-1-PBS targets POLR3C in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag27311

Product name: Recombinant human POLR3C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 196-397 aa of BC002586

Sequence: IGKGKRRRSSDEDAAGEPKAKRPKYTTDNKEPIPDDGIYWQANLDRFHQHFRDQAIVSAVANRMDQTSSEIVRTMLRMSEITTSSSAPFTQPLSSNEIFRSLPVGYNISKQVLDQYLTLLADDPLEFVGKSGDSGGGMYVINLHKALASLATATLESVVQERFGSRCARIFRLVLQKKHIEQKQVEDFAMIPAKEAKDMLYK

相同性解析による交差性が予測される生物種
Full Name polymerase (RNA) III (DNA directed) polypeptide C (62kD)
Calculated molecular weight 61 kDa
Observed molecular weight58 kDa
GenBank accession numberBC002586
Gene Symbol POLR3C
Gene ID (NCBI) 10623
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BUI4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Mutations in the POLR3A, POLR3C, POLR3E, and POLR3F subunits are associated with susceptibility to varicella zoster virus-induced encephalitis and pneumonitis (PMID: 34395528).

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