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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POLR3A Polyclonal antibody (28842-1-AP)

    WB analysis using 28842-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28842-1-AP (POLR3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using POLR3A Polyclonal antibody (28842-1-AP)

    IF Staining of A431 using 28842-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using POLR3A antibody (28842-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

POLR3A Polyclonal antibody

POLR3A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28842-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

RNA polymerase III subunit C1, hRPC155, EC:2.7.7.6, DNA-directed RNA polymerase III subunit RPC1, DNA-directed RNA polymerase III subunit A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, HeLa cells, MCF-7 cells, Y79 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28842-1-AP targets POLR3A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLR3A fusion protein Ag29036 相同性解析による交差性が予測される生物種
Full Name polymerase (RNA) III (DNA directed) polypeptide A, 155kDa
Calculated molecular weight 1390 aa, 156 kDa
Observed molecular weight155 kDa
GenBank accession numberBC041089
Gene Symbol POLR3A
Gene ID (NCBI) 11128
RRIDAB_3669672
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14802
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

POLR3A encodes the largest subunit of human RNA polymerase III (RPC1), which contributes to the catalytic activity of Pol III and is part of the active centre of the polymerase. Pol III is responsible for the transcription of untranslated RNAs (PMID: 25339210). Bi-allelic missense variants in POLR3A have been associated with phenotypes distinct from WRS: hypogonadotropic hypogonadism and hypomyelinating leukodystrophy with or without oligodontia (PMID: 30414627).

Protocols

Product Specific Protocols
WB protocol for POLR3A antibody 28842-1-APDownload protocol
IF protocol for POLR3A antibody 28842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols