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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POLR2I Polyclonal antibody (17270-1-AP)

    WB analysis using 17270-1-AP (same clone as 17270-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17270-1-AP (POLR2I antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using POLR2I Polyclonal antibody (17270-1-AP)

    IHC staining of mouse stomach using 17270-1-AP (same clone as 17270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using POLR2I Polyclonal antibody (17270-1-AP)

    IHC staining of mouse stomach using 17270-1-AP (same clone as 17270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using POLR2I Polyclonal antibody (17270-1-AP)

    IHC staining of mouse brain using 17270-1-AP (same clone as 17270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using POLR2I Polyclonal antibody (17270-1-AP)

    IHC staining of mouse brain using 17270-1-AP (same clone as 17270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using POLR2I Polyclonal antibody (17270-1-AP)

    IHC staining of rat liver using 17270-1-AP (same clone as 17270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using POLR2I Polyclonal antibody (17270-1-AP)

    IHC staining of rat liver using 17270-1-AP (same clone as 17270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using POLR2I Polyclonal antibody (17270-1-AP)

    IF Staining of HepG2 using 17270-1-AP (same clone as 17270-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POLR2I antibody (17270-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

POLR2I Polyclonal antibody, PBS Only

POLR2I Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 17270-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DNA-directed RNA polymerase II subunit I, DNA-directed RNA polymerase II subunit RPB9, hRPB14.5, RPB14.5, RPB9

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17270-1-PBS targets POLR2I in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11027

Product name: Recombinant human POLR2I protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-125 aa of BC017112

Sequence: MEPDGTYEPGFVGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE

相同性解析による交差性が予測される生物種
Full Name polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
Calculated molecular weight 125 aa, 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC017112
Gene Symbol POLR2I
Gene ID (NCBI) 5438
RRIDAB_10836936
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP36954
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

POLR2I, also known as RPB9 or hRPB14.5, is a small core subunit of RNA polymerase II involved in DNA repair and many transcription-related processes (PMID: 21450810). It is expressed in many tissues and may play a key role in the pathological process of hypertensive nephropathy (PMID: 34422001).

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