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Species Reactivity

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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using POLR2G Polyclonal antibody (18497-1-AP)

    WB analysis of A431 using 18497-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 18497-1-AP (POLR2G antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using POLR2G Polyclonal antibody (18497-1-AP)

    WB analysis using 18497-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18497-1-AP (POLR2G antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using POLR2G Polyclonal antibody (18497-1-AP)

    WB analysis of Jurkat using 18497-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 18497-1-AP (POLR2G antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using POLR2G Polyclonal antibody (18497-1-AP)

    WB analysis of A375 using 18497-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 18497-1-AP (POLR2G antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

POLR2G Polyclonal antibody

POLR2G Polyclonal Antibody for WB, ELISA
Cat No. 18497-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

hRPB19, hsRPB7, POLR2G, RNA polymerase II subunit B7, RPB19, RPB7

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, A375 cells, Jurkat cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18497-1-AP targets POLR2G in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13338

Product name: Recombinant human POLR2G protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-172 aa of BC112164

Sequence: MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQPGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFDPNSNPPCYKTMDEDIVIQQDDEIRLKIVGTRVDKNDIFAIGSLMDDYLGLVS

相同性解析による交差性が予測される生物種
Full Name polymerase (RNA) II (DNA directed) polypeptide G
Calculated molecular weight 172 aa, 19 kDa
Observed molecular weight 20-25 kDa
GenBank accession numberBC112164
Gene Symbol POLR2G
Gene ID (NCBI) 5436
RRIDAB_3085564
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP62487
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

POLR2G (DNA- directed RNA polymerase II subunit G), also known as hRPB19, hsRPB7 and RPB7, is the seventh largest subunit of RNA polymerase II. POLR2G functions in transcription initiation, and is also thought to help stabilize transcribing polyermase molecules during elongation.

Protocols

Product Specific Protocols
WB protocol for POLR2G antibody 18497-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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