Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 16093-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16093-1-AP (POLR2D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 16093-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16093-1-AP (POLR2D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 16093-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16093-1-AP (POLR2D antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 16093-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 16093-1-AP (POLR2D antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of rat skeletal muscle using 16093-1-AP
rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16093-1-AP (POLR2D antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 16093-1-AP
IP result of anti-POLR2D (IP:16093-1-AP, 4ug; Detection:16093-1-AP 1:800) with mouse heart tissue lysate 4000ug.
IHC staining of mouse testis using 16093-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16093-1-AP (POLR2D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 16093-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16093-1-AP (POLR2D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

POLR2D Polyclonal antibody

POLR2D Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 16093-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

DNA-directed RNA polymerase II subunit D, DNA-directed RNA polymerase II subunit RPB4, HSRBP4, HSRPB4, RNA polymerase II RPB4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, rat liver tissue, rat skeletal muscle tissue, HepG2 cells, MCF-7 cells
Positive IP detected in	mouse heart tissue
Positive IHC detected in	mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below

Product Information

16093-1-AP targets POLR2D in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8701 Product name: Recombinant human POLR2D protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-142 aa of BC017205 Sequence: MAAGGSDPRAGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGRFEDEELQQILDDIQTKRSFQY 相同性解析による交差性が予測される生物種
Full Name	polymerase (RNA) II (DNA directed) polypeptide D
Calculated molecular weight	142 aa, 16 kDa
Observed molecular weight	16-20 kDa
GenBank accession number	BC017205
Gene Symbol	POLR2D
Gene ID (NCBI)	5433
RRID	AB_2284144
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O15514
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for POLR2D antibody 16093-1-AP	Download protocol
IP protocol for POLR2D antibody 16093-1-AP	Download protocol
WB protocol for POLR2D antibody 16093-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Mol Cell RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits Authors - Yuanjun Li View Article
mouse	WB	Mol Cell Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity Authors - Yuanjun Li View Article
human	WB	Elife A cryptic K48 ubiquitin chain binding site on UCH37 is required for its role in proteasomal degradation. Authors - Jiale Du View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16093-1-AP

WB analysis using 16093-1-AP

WB analysis using 16093-1-AP

WB analysis of rat liver using 16093-1-AP

WB analysis of rat skeletal muscle using 16093-1-AP

IP experiment of mouse heart using 16093-1-AP

IHC staining of mouse testis using 16093-1-AP

IHC staining of mouse testis using 16093-1-AP

POLR2D Polyclonal antibody

POLR2D Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits

Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity

A cryptic K48 ubiquitin chain binding site on UCH37 is required for its role in proteasomal degradation.