Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 16145-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16145-1-AP (POLR1E antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 16145-1-AP
WB result of POLR1E antibody (16145-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-POLR1E transfected MCF-7 cells.
WB analysis of human brain using 16145-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 16145-1-AP (POLR1E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 16145-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16145-1-AP (POLR1E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 16145-1-AP
IP result of anti-POLR1E (IP:16145-1-AP, 3ug; Detection:16145-1-AP 1:500) with Jurkat cells lysate 1000ug.
IHC staining of human liver cancer using 16145-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16145-1-AP (POLR1E Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

POLR1E Polyclonal antibody

POLR1E Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 16145-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ChIP, ELISA

Synonyms

PAF53, POLR1E, PRAF1, RNA polymerase I subunit A49, RP11 405L18.3

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (7)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, human brain tissue, Jurkat cells, MCF-7 cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IF	See 3 publications below
ChIP	See 1 publications below

Product Information

16145-1-AP targets POLR1E in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	POLR1E fusion protein Ag9269 相同性解析による交差性が予測される生物種
Full Name	polymerase (RNA) I polypeptide E, 53kDa
Calculated molecular weight	481aa,54 kDa; 419aa,47 kDa
Observed molecular weight	47-53 kDa
GenBank accession number	BC014331
Gene Symbol	POLR1E
Gene ID (NCBI)	64425
RRID	AB_2167341
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9GZS1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for POLR1E antibody 16145-1-AP	Download protocol
IHC protocol for POLR1E antibody 16145-1-AP	Download protocol
IP protocol for POLR1E antibody 16145-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	ChIP	Science lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription. Authors - Man Wu View Article
human	WB	J Biol Chem Identification of an E3 ligase that targets the catalytic subunit of RNA polymerase I upon transcription stress Authors - Stephanie Pitts View Article
	WB	J Biol Chem Conditional depletion of the RNA polymerase I subunit PAF53 reveals that it is essential for mitosis and enables identification of functional domains. Authors - Rachel McNamar View Article
human	WB	Cancer Invest Quinacrine Depletes BCR-ABL and Suppresses Ph-Positive Leukemia Cells Authors - Hu Lei View Article
human	IF	PLoS One Expression of RNA polymerase I catalytic core is influenced by RPA12 Authors - Brittany L Ford View Article
human	IF	Mol Biol Cell Nucleoli and the nucleoli-centromere association are dynamic during normal development and in cancer Authors - Aaron Rodrigues View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16145-1-AP

WB analysis of MCF-7 using 16145-1-AP

WB analysis of human brain using 16145-1-AP

WB analysis of Jurkat using 16145-1-AP

IP experiment of Jurkat using 16145-1-AP

IHC staining of human liver cancer using 16145-1-AP

POLR1E Polyclonal antibody

POLR1E Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription.

Identification of an E3 ligase that targets the catalytic subunit of RNA polymerase I upon transcription stress

Conditional depletion of the RNA polymerase I subunit PAF53 reveals that it is essential for mitosis and enables identification of functional domains.

Quinacrine Depletes BCR-ABL and Suppresses Ph-Positive Leukemia Cells

Expression of RNA polymerase I catalytic core is influenced by RPA12

Nucleoli and the nucleoli-centromere association are dynamic during normal development and in cancer