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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

POLR1D Polyclonal antibody

POLR1D Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 16678-1-AP

Synonyms

AC19, hRPA19, POLR1C, POLR1D, RPA16, RPA9, RPAC2, RPC16, RPO1 3

Validation Data Gallery

Applications:
  • WB analysis of Jurkat using 16678-1-AP

    WB analysis of Jurkat using 16678-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16678-1-AP (POLR1D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 16678-1-AP

    WB analysis of HeLa using 16678-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16678-1-AP (POLR1D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 16678-1-AP

    WB analysis of HepG2 using 16678-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16678-1-AP (POLR1D Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16678-1-AP

    IF Staining of HepG2 using 16678-1-AP

    Immunofluorescent analysis of HepG2 cells using 16678-1-AP (POLR1D antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 16678-1-AP is POLR1D Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLR1D fusion protein Ag10000 相同性解析による交差性が予測される生物種
Full Name polymerase (RNA) I polypeptide D, 16kDa
Calculated molecular weight 15 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC000889
Gene symbol POLR1D
Gene ID (NCBI) 51082
RRIDAB_2878298
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for POLR1D antibody 16678-1-APDownload protocol
IF protocol for POLR1D antibody 16678-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols