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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using POLR1D Polyclonal antibody (12254-1-AP)

    WB analysis of HeLa using 12254-1-AP (same clone as 12254-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12254-1-AP (POLR1D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12254-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using POLR1D Polyclonal antibody (12254-1-AP)

    WB analysis of Jurkat using 12254-1-AP (same clone as 12254-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12254-1-AP (POLR1D antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12254-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using POLR1D Polyclonal antibody (12254-1-AP)

    WB analysis of mouse lung using 12254-1-AP (same clone as 12254-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 12254-1-AP (POLR1D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12254-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using POLR1D Polyclonal antibody (12254-1-AP)

    IP experiment of HeLa using 12254-1-AP (same clone as 12254-1-PBS)

    IP result of anti-POLR1D (IP:12254-1-AP, 4ug; Detection:12254-1-AP 1:500) with HeLa cells lysate 2600 ug. This data was developed using the same antibody clone with 12254-1-PBS in a different storage buffer formulation.

POLR1D Polyclonal antibody, PBS Only

POLR1D Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 12254-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12254-1-PBS targets POLR1D in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2904

Product name: Recombinant human POLR1D protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-122 aa of BC015319

Sequence: MEEDQELERKAIEELLKEAKRGKTRAETMGPMGWMKCPLASTNKRFLINTIKNTLPSHKEQDHEQKEGDKEPAKSQAQKEENPKKHRSHPYKHSFRARGSASYSPPRKRSSQDKYEKRSNRR

相同性解析による交差性が予測される生物種
Full Name polymerase (RNA) I polypeptide D, 16kDa
Calculated molecular weight 16 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC015319
Gene Symbol POLR1D
Gene ID (NCBI) 51082
RRIDAB_10640535
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP0DPB6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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