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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POLL Polyclonal antibody (29920-1-AP)

    WB analysis using 29920-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29920-1-AP (POLL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using POLL Polyclonal antibody (29920-1-AP)

    WB analysis using 29920-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29920-1-AP (POLL antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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POLL Polyclonal antibody

POLL Polyclonal Antibody for WB, ELISA
Cat No. 29920-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

BETA N, DNA polymerase beta 2, DNA polymerase kappa, DNA polymerase lambda, FLJ46002, Pol beta2, POL KAPPA, Pol Lambda, POLL

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inL02 cells, HEK-293 cells, HepG2 cells, HEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29920-1-AP targets POLL in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLL fusion protein Ag31995 相同性解析による交差性が予測される生物種
Full Name polymerase (DNA directed), lambda
Calculated molecular weight 63 kDa
Observed molecular weight63-70 kDa
GenBank accession numberBC068529
Gene Symbol POLL
Gene ID (NCBI) 27343
RRIDAB_3086189
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UGP5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

POLL (DNA polymerase lambda) functions in DNA repair (PMID: 11457865, PMID: 19806195, PMID: 20693240, PMID: 30250067). POLL has 5-prime-deoxyribose-5-phosphate lyase activity and strand-displacement synthesis activity on gapped DNA substrates, indicating that POLL participates in short- and long-patch base excision repair. POLL is involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA (PMID: 11457865, PMID: 19806195).

Protocols

Product Specific Protocols
WB protocol for POLL antibody 29920-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols