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Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using POLH Polyclonal antibody (28133-1-AP)

    WB analysis of BxPC-3 using 28133-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 28133-1-AP (POLH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using POLH Polyclonal antibody (28133-1-AP)

    WB analysis of HeLa using 28133-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 28133-1-AP (POLH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using POLH Polyclonal antibody (28133-1-AP)

    WB analysis of COLO 320 using 28133-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 28133-1-AP (POLH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using POLH Polyclonal antibody (28133-1-AP)

    WB analysis of HEK-293T using 28133-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 28133-1-AP (POLH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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POLH Polyclonal antibody

POLH Polyclonal Antibody for WB, ELISA
Cat No. 28133-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, ELISA

Synonyms

DNA polymerase eta, FLJ16395, FLJ21978, POLH, polymerase (DNA directed), eta, RAD30, RAD30 homolog A, RAD30A, XP V, XPV

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells, COLO 320 cells, HEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28133-1-AP targets POLH in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLH fusion protein Ag27973 相同性解析による交差性が予測される生物種
Full Name polymerase (DNA directed), eta
Calculated molecular weight713aa,78 kDa; 414aa,46 kDa
Observed molecular weight 78-85 kDa
GenBank accession numberBC015742
Gene Symbol POLH
Gene ID (NCBI) 5429
RRIDAB_2881070
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y253
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for POLH antibody 28133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

J Tradit Chin Med

Integrating network pharmacology and experimental validation to uncover the synergistic effects of Huangqi ()-Ezhu () with 5-fluorouracil in colorectal cancer models

Authors - Tan Xiying
View Article