Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, H9C2 cells, mouse colon tissue, rat colon tissue, COLO 320 cells, A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85852-4-RR targets POLG2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | POLG2 fusion protein Ag1424 相同性解析による交差性が予測される生物種 |
| Full Name | polymerase (DNA directed), gamma 2, accessory subunit |
| Calculated molecular weight | 55 kDa |
| Observed molecular weight | 52-55 kDa |
| GenBank accession number | BC009194 |
| Gene Symbol | POLG2 |
| Gene ID (NCBI) | 11232 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UHN1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
by POLG) and an accessory subunit (encoded by POLG2). POLG2(DNA polymerase subunit gamma-2, mitochondrial) can stimulate the polymerase and exonuclease activities (PMID: 37085601). Defects in POLG2 are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 4 (PEOA4) (PMID: 16685652).Western blot result suggested that the POLG2 expressed in human fetal kidney and U-251 MG cell lines with a single band of 55kd.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for POLG2 antibody 85852-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |