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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POLG Recombinant antibody (83670-1-RR)

    WB analysis using 83670-1-RR (same clone as 83670-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83670-1-RR (POLG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83670-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat testis tissue using POLG Recombinant antibody (83670-1-RR)

    WB analysis of rat testis using 83670-1-RR (same clone as 83670-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 83670-1-RR (POLG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83670-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using POLG Recombinant antibody (83670-1-RR)

    WB analysis of rat brain using 83670-1-RR (same clone as 83670-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 83670-1-RR (POLG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83670-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83670-1-RR

    Affinity and Kinetic Characterization of 83670-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83670-1-RR against Human POLG were performed. The affinity constant is below 1 pM.

POLG Recombinant monoclonal antibody, PBS Only

POLG Uni-rAb® Recombinant Antibody for ELISA, WB
Cat No. 83670-1-PBS
Clone No.240441D2

Host / Isotype

Rabbit / IgG

Reactivity

rat, human

Applications

ELISA, WB

Synonyms

EC:3.1.11.-, EC:2.7.7.7, DNA polymerase subunit gamma-1, 5'-deoxyribose-phosphate lyase, 3'-5' exodeoxyribonuclease

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83670-1-PBS targets POLG in ELISA, WB applications and shows reactivity with rat, human samples.

Tested Reactivity rat, human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag5256

Product name: Recombinant human POLG protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 888-1239 aa of BC042571

Sequence: GADVDSQELWIAAVLGDAHFAGMHGCTAFGWMTLQGRKSRGTDLHSKTATTVGISREHAKIFNYGRIYGAGQPFAERLLMQFNHRLTQQEAAEKAQQMYAATKGLRWYRLSDEGEWLVRELNLPVDRTEGGWISLQDLRKVQRETARKSQWKKWEVVAERAWKGGTESEMFNKLESIATSDIPRTPVLGCCISRALEPSAVQEEFMTSRVNWVVQSSAVDYLHLMLVAMKWLFEEFAIDGRFCISIHDEVRYLVREEDRYRAALALQITNLLTRCMFAYKLGLNDLPQSVAFFSAVDIDRCLRKEVTMDCKTPSNPTGMERRYGIPQGEALDIYQIIELTKGSLEKRSQPGP

相同性解析による交差性が予測される生物種
Full Name polymerase (DNA directed), gamma
Calculated molecular weight 140 kDa
GenBank accession numberBC042571
Gene Symbol POLG
Gene ID (NCBI) 5428
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP54098
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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