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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POLG Polyclonal antibody (14110-1-AP)

    WB analysis using 14110-1-AP (same clone as 14110-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14110-1-AP (POLG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14110-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293T cells using POLG Polyclonal antibody (14110-1-AP)

    WB analysis of HEK-293T using 14110-1-AP (same clone as 14110-1-PBS)

    WB result of POLG antibody (14110-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-POLG transfected HEK-293T cells. This data was developed using the same antibody clone with 14110-1-PBS in a different storage buffer formulation.

POLG Polyclonal antibody, PBS Only

POLG Polyclonal Antibody for WB, Indirect ELISA
Cat No. 14110-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

3'-5' exodeoxyribonuclease, 5'-deoxyribose-phosphate lyase, DNA polymerase subunit gamma-1, EC:2.7.7.7, EC:3.1.11.-

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14110-1-PBS targets POLG in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5256

Product name: Recombinant human POLG protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 888-1239 aa of BC042571

Sequence: GADVDSQELWIAAVLGDAHFAGMHGCTAFGWMTLQGRKSRGTDLHSKTATTVGISREHAKIFNYGRIYGAGQPFAERLLMQFNHRLTQQEAAEKAQQMYAATKGLRWYRLSDEGEWLVRELNLPVDRTEGGWISLQDLRKVQRETARKSQWKKWEVVAERAWKGGTESEMFNKLESIATSDIPRTPVLGCCISRALEPSAVQEEFMTSRVNWVVQSSAVDYLHLMLVAMKWLFEEFAIDGRFCISIHDEVRYLVREEDRYRAALALQITNLLTRCMFAYKLGLNDLPQSVAFFSAVDIDRCLRKEVTMDCKTPSNPTGMERRYGIPQGEALDIYQIIELTKGSLEKRSQPGP

相同性解析による交差性が予測される生物種
Full Name polymerase (DNA directed), gamma
Calculated molecular weight 140 kDa
Observed molecular weight 130-150 kDa
GenBank accession numberBC042571
Gene Symbol POLG
Gene ID (NCBI) 5428
RRIDAB_3669178
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP54098
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

POLG is the catalytic subunit of DNA polymerase gamma solely responsible for the replication of mitochondrial DNA (mtDNA). POLG replicates both heavy and light strands of the circular mtDNA genome using a single-stranded DNA template, RNA primers, and the four deoxyribonucleoside triphosphates as substrates (PMID:11477093; 11897778; 15917273; 19837034; 9558343).

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