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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POLD2 Polyclonal antibody (29765-1-AP)

    WB analysis using 29765-1-AP (same clone as 29765-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29765-1-AP (POLD2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using POLD2 Polyclonal antibody (29765-1-AP)

    WB analysis using 29765-1-AP (same clone as 29765-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29765-1-AP (POLD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using POLD2 Polyclonal antibody (29765-1-AP)

    WB analysis using 29765-1-AP (same clone as 29765-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29765-1-AP (POLD2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using POLD2 Polyclonal antibody (29765-1-AP)

    IHC staining of human colon using 29765-1-AP (same clone as 29765-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using POLD2 Polyclonal antibody (29765-1-AP)

    IHC staining of human colon using 29765-1-AP (same clone as 29765-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using POLD2 Polyclonal antibody (29765-1-AP)

    IHC staining of human lung cancer using 29765-1-AP (same clone as 29765-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using POLD2 Polyclonal antibody (29765-1-AP)

    IHC staining of human lung cancer using 29765-1-AP (same clone as 29765-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using POLD2 Polyclonal antibody (29765-1-AP)

    IF Staining of HeLa using 29765-1-AP (same clone as 29765-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLD2 antibody (29765-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

POLD2 Polyclonal antibody, PBS Only

POLD2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29765-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DNA polymerase delta subunit 2

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29765-1-PBS targets POLD2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag31521

Product name: Recombinant human POLD2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 40-195 aa of BC000459

Sequence: RLGERSFSRQYAHIYATRLIQMRPFLENRAQQHWGSGVGVKKLCELQPEEKCCVVGTLFKAMPLQPSILREVSEEHNLLPQPPRSKYIHPDDELVLEDELQRIKLKGTIDVSKLVTGTVLAVFGSVRDDGKFLVEDYCFADLAPQKPAPPLDTDRF

相同性解析による交差性が予測される生物種
Full Name polymerase (DNA directed), delta 2, regulatory subunit 50kDa
Calculated molecular weight 51 kDa
Observed molecular weight48-51 kDa
GenBank accession numberBC000459
Gene Symbol POLD2
Gene ID (NCBI) 5425
RRIDAB_2918344
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP49005
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

POLD2, also named as DNA polymerase delta subunit 2, is a 469 amino acid protein, which belongs to the DNA polymerase delta/II small subunit family. The function of the small subunit is not yet clear. POLD2 has an essential function for the small subunit in the heterodimeric core enzyme.

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