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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Multi-rAb® POLD1 Multi-Recombinant antibody (RMX00101)

    WB analysis using RMX00101

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00101 (POLD1 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using Multi-rAb® POLD1 Multi-Recombinant antibody (RMX00101)

    IHC staining of human colon using RMX00101

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using RMX00101 (POLD1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Multi-rAb® POLD1 Multi-Recombinant antibody (RMX00101)

    IHC staining of human colon using RMX00101

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using RMX00101 (POLD1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Multi-rAb® POLD1 Multi-Recombinant antibody (RMX00101)

    IF Staining of HeLa using RMX00101

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLD1 antibody (RMX00101) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Multi-rAb® POLD1 Multi-Recombinant antibody

POLD1 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. RMX00101

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

3'-5' exodeoxyribonuclease, CDC2, EC:2.7.7.7, EC:3.1.11.-, POLD

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, PC-12 cells, RAW 264.7 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

RMX00101 targets POLD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Multi-Recombinant
Type Antibody
Immunogen

Recombinant protein

相同性解析による交差性が予測される生物種
Full Name polymerase (DNA directed), delta 1, catalytic subunit 125kDa
Calculated molecular weight 1107 aa, 124 kDa
Observed molecular weight 124 kDa
GenBank accession numberBC008800
Gene Symbol POLD1
Gene ID (NCBI) 5424
Conjugate Unconjugated
Form
FormLiquid
Purification MethodN/A
UNIPROT IDP28340
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for POLD1 antibody RMX00101Download protocol
IHC protocol for POLD1 antibody RMX00101Download protocol
WB protocol for POLD1 antibody RMX00101Download protocol
Standard Protocols
Click here to view our Standard Protocols
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