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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POLD1 Recombinant antibody (86197-2-RR)

    WB analysis using 86197-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86197-2-RR (POLD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using POLD1 Recombinant monoclonal antibody (86197-2-RR)

    IHC staining of human colon cancer using 86197-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86197-2-RR (POLD1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using POLD1 Recombinant antibody (86197-2-RR)

    IF Staining of HeLa using 86197-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLD1 antibody (86197-2-RR, Clone: 250828E5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86197-2-RR

    Affinity and Kinetic Characterization of 86197-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86197-2-RR against Human POLD1 were performed. The affinity constant is below 1 pM.

POLD1 Recombinant monoclonal antibody

POLD1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 86197-2-RR
Clone No.250828E5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

3'-5' exodeoxyribonuclease, CDC2, EC:2.7.7.7, EC:3.1.11.-, POLD

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, RAW 264.7 cells, PC-12 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86197-2-RR targets POLD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag8178

Product name: Recombinant human POLD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 858-1107 aa of BC008800

Sequence: EGAVAHAQDVISDLLCNRIDISQLVITKELTRAASDYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIISAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAEAVLLRGDHTRCKTVLTGKVGGLLAFAKRRNCCIGCRTVLSHQGAVCEFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQEQLLRRFGPPGPEAW

相同性解析による交差性が予測される生物種
Full Name polymerase (DNA directed), delta 1, catalytic subunit 125kDa
Calculated molecular weight 1107 aa, 124 kDa
Observed molecular weight 124 kDa
GenBank accession numberBC008800
Gene Symbol POLD1
Gene ID (NCBI) 5424
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP28340
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

POLD1, also known as DNA polymerase subunit delta p125, is 1107 amino acid protein, which belongs to the DNA polymerase type-B family. POLD1 colocalizes with PCNA and POLD3 at S phase replication sites (PubMed:11595739). As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. A previous study demonstrated that overexpression of POLD1 is associated with an increased risk of breast cancer. (

Protocols

Product Specific Protocols
IF protocol for POLD1 antibody 86197-2-RRDownload protocol
IHC protocol for POLD1 antibody 86197-2-RRDownload protocol
WB protocol for POLD1 antibody 86197-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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