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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POLB Recombinant monoclonal antibody (86680-2-RR)

    WB analysis using 86680-2-RR (same clone as 86680-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86680-2-RR (POLB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86680-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of COLO 320 cells using POLB Recombinant monoclonal antibody (86680-2-RR)

    IF Staining of COLO 320 using 86680-2-RR (same clone as 86680-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using POLB antibody (86680-2-RR, Clone: 251689E10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86680-2-PBS in a different storage buffer formulation.

POLB Recombinant monoclonal antibody, PBS Only

POLB Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86680-2-PBS
Clone No.251689E10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

5'-deoxyribose-phosphate lyase, 5'-dRP lyase, AP lyase, DNA polymerase beta, EC:2.7.7.7

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86680-2-PBS targets POLB in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag12496

Product name: Recombinant human POLB protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-335 aa of BC100288

Sequence: MSKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSKGETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE

相同性解析による交差性が予測される生物種
Full Name polymerase (DNA directed), beta
Calculated molecular weight 335 aa, 38 kDa
Observed molecular weight40 kDa
GenBank accession numberBC100288
Gene Symbol DNA Polymerase Beta
Gene ID (NCBI) 5423
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP06746
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DNA polymerase β (DNA polymerase beta (POLB)) belongs to a member of the DNA polymerase X family, mainly involved in various biological metabolic processes, such as eukaryotic DNA replication, DNA damage repair, gene recombination, and cell cycle regulation (PMID: 38731327).

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