Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
86856-1-PBS targets POLA2 as part of a matched antibody pair:
MP02735-1: 86856-1-PBS capture and 86856-2-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag0271 Product name: Recombinant human POLA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 17-275 aa of BC001347 Sequence: LDCEEALIEKLVELCVQYGQNEEGMVGELIAFCTSTHKVGLTSEILNSFEHEFLSKRLSKARHSTCKDSGHAGARDIVSIQELIEVEEEEEILLNSYTTPSKGSQKRAISTPETPLTKRSVSTRSPHQLLSPSSFSPSATPSQKYNSRSNRGEVVTSFGLAQGVSWSGRGGAGNISLKVLGCPEALTGSYKSMFQKLPDIREVLTCKIEELGSELKEHYKIEAFTPLLAPAQEPVTLLGQIGCDSNGKLNNKSVILEGD 相同性解析による交差性が予測される生物種 |
| Full Name | polymerase (DNA directed), alpha 2 (70kD subunit) |
| Calculated molecular weight | 70 kDa |
| Observed molecular weight | 68-70 kDa |
| GenBank accession number | BC001347 |
| Gene Symbol | POLA2 |
| Gene ID (NCBI) | 23649 |
| RRID | AB_3745166 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14181 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
POLA2 (DNA polymerase α subunit B) is the non-catalytic regulatory subunit of DNA polymerase α (Polα-primase complex). It connects the catalytic subunit POLA1 to the primase subunits PRIM1/PRIM2, stabilizing the tetrameric complex and ensuring primer synthesis and translocation to the polymerase active site to initiate DNA replication. This protein is expressed in the cell nucleus and is crucial for DNA replication and cell cycle progression in eukaryotes.