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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using POLA2 Polyclonal antibody (21778-1-AP)

    WB analysis of HepG2 using 21778-1-AP (same clone as 21778-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using POLA2 Polyclonal antibody (21778-1-AP)

    WB analysis of COLO 320 using 21778-1-AP (same clone as 21778-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using POLA2 Polyclonal antibody (21778-1-AP)

    WB analysis of HeLa using 21778-1-AP (same clone as 21778-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using POLA2 Polyclonal antibody (21778-1-AP)

    WB analysis of COLO 320 using 21778-1-AP (same clone as 21778-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using POLA2 Polyclonal antibody (21778-1-AP)

    WB analysis of MCF-7 using 21778-1-AP (same clone as 21778-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using POLA2 Polyclonal antibody (21778-1-AP)

    WB analysis of HepG2 using 21778-1-AP (same clone as 21778-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using POLA2 Polyclonal antibody (21778-1-AP)

    WB analysis of HeLa using 21778-1-AP (same clone as 21778-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using POLA2 Polyclonal antibody (21778-1-AP)

    WB analysis of HeLa using 21778-1-AP (same clone as 21778-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using POLA2 Polyclonal antibody (21778-1-AP)

    IF Staining of HeLa using 21778-1-AP (same clone as 21778-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 21778-1-AP (POLA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.

POLA2 Polyclonal antibody, PBS Only

POLA2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 21778-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21778-1-PBS targets POLA2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0271

Product name: Recombinant human POLA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 17-275 aa of BC001347

Sequence: LDCEEALIEKLVELCVQYGQNEEGMVGELIAFCTSTHKVGLTSEILNSFEHEFLSKRLSKARHSTCKDSGHAGARDIVSIQELIEVEEEEEILLNSYTTPSKGSQKRAISTPETPLTKRSVSTRSPHQLLSPSSFSPSATPSQKYNSRSNRGEVVTSFGLAQGVSWSGRGGAGNISLKVLGCPEALTGSYKSMFQKLPDIREVLTCKIEELGSELKEHYKIEAFTPLLAPAQEPVTLLGQIGCDSNGKLNNKSVILEGD

相同性解析による交差性が予測される生物種
Full Name polymerase (DNA directed), alpha 2 (70kD subunit)
Calculated molecular weight 70 kDa
Observed molecular weight 68-70 kDa
GenBank accession numberBC001347
Gene Symbol POLA2
Gene ID (NCBI) 23649
RRIDAB_10733243
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14181
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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