Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, LNCap cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
| Positive IHC detected in | human stomach cancer tissue, mouse stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HepG2 cells, LNCaP cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.2 μg per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68309-1-Ig targets PNPT1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6290 Product name: Recombinant human PNPT1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 427-783 aa of BC053660 Sequence: YEFPPYATNEIGKVTGLNRRELGHGALAEKALYPVIPRDFPFTIRVTSEVLESNGSSSMASACGGSLALMDSGVPISSAVAGVAIGLVTKTDPEKGEIEDYRLLTDILGIEDYNGDMDFKIAGTNKGITALQADIKLPGIPIKIVMEAIQQASVAKKEILQIMNKTISKPRASRKENGPVVETVQVPLSKRAKFVGPGGYNLKKLQAETGVTISQVDEETFSVFAPTPSAMHEARDFITEICKDDQEQQLEFGAVYTATITEIRDTGVMVKLYPNMTAVLLHNTQLDQRKIKHPTALGLEVGQEIQVKYFGRDPADGRMRLSRKVLQSPATTVVRTLNDRSSIVMGEPISQSSSNSQ 相同性解析による交差性が予測される生物種 |
| Full Name | polyribonucleotide nucleotidyltransferase 1 |
| Calculated molecular weight | 86 kDa |
| Observed molecular weight | 86 kDa |
| GenBank accession number | BC053660 |
| Gene Symbol | PNPT1 |
| Gene ID (NCBI) | 87178 |
| RRID | AB_2935388 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8TCS8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PNPT1 (polynucleotide phosphorylase1; also known as PNPASE) localizes in the mitochondrial intermembrane and regulates RNA import into mitochondria (20691904). PNPT1 is also involved in mRNA degradation. As a type I IFN-inducible gene, PNPT1 plays an essential role in mediating IFN-mediated inflammatory processes (17804700). Recently mutation in PNPT1 has been reported to cause hereditary hearing loss (23084290). This antibody detected the endogenous PNPT1 around 80 kDa in mouse brain.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PNPT1 antibody 68309-1-Ig | Download protocol |
| IHC protocol for PNPT1 antibody 68309-1-Ig | Download protocol |
| WB protocol for PNPT1 antibody 68309-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |