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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PNPLA6 Polyclonal antibody (14261-1-AP)

    WB analysis of HEK-293 using 14261-1-AP (same clone as 14261-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14261-1-AP (PNPLA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14261-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using PNPLA6 Polyclonal antibody (14261-1-AP)

    WB analysis of SH-SY5Y using 14261-1-AP (same clone as 14261-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14261-1-AP (PNPLA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14261-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment using PNPLA6 Polyclonal antibody (14261-1-AP)

    IP experiment using 14261-1-AP (same clone as 14261-1-PBS)

    IP result of anti-PNPLA6 (14261-1-AP for IP and Detection) with HEK-293 cells. This data was developed using the same antibody clone with 14261-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using PNPLA6 Polyclonal antibody (14261-1-AP)

    IHC staining of rat brain using 14261-1-AP (same clone as 14261-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14261-1-AP (PNPLA6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14261-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PNPLA6 Polyclonal antibody (14261-1-AP)

    IF Staining of HepG2 using 14261-1-AP (same clone as 14261-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using PNPLA6 antibody 14261-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 14261-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PNPLA6 Polyclonal antibody (14261-1-AP)

    IF Staining of MCF-7 using 14261-1-AP (same clone as 14261-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using PNPLA6 antibody 14261-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14261-1-PBS in a different storage buffer formulation.

PNPLA6 Polyclonal antibody, PBS Only

PNPLA6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14261-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

EC:3.1.1.5, NTE, NTEMND, Patatin-like phospholipase domain-containing protein 6, SPG39

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14261-1-PBS targets PNPLA6 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5546

Product name: Recombinant human PNPLA6 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 977-1327 aa of BC051768

Sequence: EERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPPIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWSRGNVIEKMLTDRRSTDLNESRRADVLAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEMEEEKSILRQRRCLPQEPPGSATDA

相同性解析による交差性が予測される生物種
Full Name patatin-like phospholipase domain containing 6
Calculated molecular weight 150 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC051768
Gene Symbol PNPLA6
Gene ID (NCBI) 10908
RRIDAB_2283965
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IY17
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PNPLA6(patatin-like phospholipase domain-containing protein 6) is also named as NTE(neuropathy target esterase) and belongs to the NTE family. It plays an important role in motor neuron development and implicated overexpression of BMP signaling(PMID:22996643). Defects in PNPLA6 are the cause of spastic paraplegia autosomal recessive type 39 (SPG39)(PMID:18313024). This protein has a glycosylaion site that it can be glycosylated(PMID:9576844). It has 3 isoforms produced by alternative splicing.

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