Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 21059-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21059-1-AP (PNO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 21059-1-AP
WB result of PNO1 antibody (21059-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-PNO1 transfected K-562 cells.
WB analysis of mouse spleen using 21059-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21059-1-AP (PNO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of L02 using 21059-1-AP
IP result of anti-PNO1 (IP:21059-1-AP, 3ug; Detection:21059-1-AP 1:300) with L02 cells lysate 1500ug.
IHC staining of human liver cancer using 21059-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21059-1-AP (PNO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 21059-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 21059-1-AP (PNO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 21059-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 21059-1-AP (PNO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

PNO1 Polyclonal antibody

PNO1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 21059-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

KHRBP1, Partner of NOB1, RNA binding protein PNO1, RNA-binding protein PNO1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration: 150 μL, 900 ug/ml

20 μL, 900 ug/ml

150 μL, 900 ug/ml

¥67,000/ 150 μL

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A2780 cells, mouse spleen tissue, K-562 cells, A549 cells
Positive IP detected in	L02 cells
Positive IHC detected in	human liver cancer tissue, human urothelial carcinoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IHC	See 2 publications below

Product Information

21059-1-AP targets PNO1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PNO1 fusion protein Ag14752 相同性解析による交差性が予測される生物種
Full Name	partner of NOB1 homolog (S. cerevisiae)
Calculated molecular weight	252 aa, 28 kDa
Observed molecular weight	28-35 kDa
GenBank accession number	BC008304
Gene Symbol	PNO1
Gene ID (NCBI)	56902
RRID	AB_10734582
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NRX1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Human RNA binding gene partner of NOB1 (PNO1) was firstly isolated from human kidney and encodes 35-kDa protein, named PNO1. PNO1 as a ribosome biogenesis factor that contributes to 40S subunit assembly by binding with its partner NOB1. PNO1 is a highly conserved protein with a K homology (KH) domain at its C-terminal and two putative nuclear localization signals at its N-terminal . PNO1 also had great regulatory function in tumor metabolism. (PMID: 38071381, PMID: 33864661)

Protocols

Product Specific Protocols
WB protocol for PNO1 antibody 21059-1-AP	Download protocol
IHC protocol for PNO1 antibody 21059-1-AP	Download protocol
IP protocol for PNO1 antibody 21059-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Sci Rep PNO1 promotes the progression of osteosarcoma via TGF-β and YAP/TAZ pathway Authors - Long Fang KD Validated View Article
human	IHC	Heliyon Pan-cancer exploration of PNO1: A prospective prognostic biomarker with ties to immune infiltration Authors - Yinhui Qin View Article
human	WB,IHC	Sci Rep PNO1 enhances ovarian cancer cell growth, invasion, and stemness via activating the AKT/Wnt/β-catenin pathway Authors - Lu Qin View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 21059-1-AP

WB analysis of K-562 using 21059-1-AP

WB analysis of mouse spleen using 21059-1-AP

IP experiment of L02 using 21059-1-AP

IHC staining of human liver cancer using 21059-1-AP

IHC staining of human urothelial carcinoma using 21059-1-AP

IHC staining of human urothelial carcinoma using 21059-1-AP

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