Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83979-5-RR targets PNMT in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag4098 Product name: Recombinant human PNMT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-282 aa of BC037246 Sequence: MSGADRSPNAGAAPDSAPGQAAVASAYQRFEPRAYLRNNYAPPRGDLCNPNGVGPWKLRCLAQTFATGEVSGRTLIDIGSGPTVYQLLSACSHFEDITMTDFLEVNRQELGRWLQEEPGAFNWSMYSQHACLIEGKGECWQDKERQLRARVKRVLPIDVHQPQPLGAGSPAPLPADALVSAFCLEAVSPDLASFQRALDHITTLLRPGGHLLLIGALEESWYLAGEARLTVVPVSEEEVREALVRSGYKVRDLRTYIMPAHLQTGVDDVKGVFFAWAQKVGL 相同性解析による交差性が予測される生物種 |
| Full Name | phenylethanolamine N-methyltransferase |
| Calculated molecular weight | 282 aa, 31 kDa |
| Observed molecular weight | 31 kDa |
| GenBank accession number | BC037246 |
| Gene Symbol | PNMT |
| Gene ID (NCBI) | 5409 |
| RRID | AB_3671553 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P11086 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PNMT (Phenylethanolamine N-methyltransferase) is the final enzyme in the catecholamine synthesizing cascade that converts noradrenaline to epinephrine by transferring a methyl group from S-adenosyl-L-methionine (SAM) to norepinephrine. PNMT is primarily found in the adrenal medulla, where it is responsible for the production of epinephrine. It is also present in certain areas of the brain, such as the amygdala and retina, where it may be involved in various neurophysiological functions (PMID: 17175506). Variations in the PNMT gene have been associated with Alzheimer's disease, hypertension, and other disorders (PMID: 2816488,.11514309).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PNMT antibody 83979-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |