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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using PNMT Polyclonal antibody (13217-1-AP)

    WB analysis of K-562 using 13217-1-AP (same clone as 13217-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 13217-1-AP (PNMT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13217-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of PC-12 cells using PNMT Polyclonal antibody (13217-1-AP)

    IP experiment of PC-12 using 13217-1-AP (same clone as 13217-1-PBS)

    IP result of anti-PNMT (IP:13217-1-AP, 4ug; Detection:13217-1-AP 1:300) with PC-12 cells lysate 2400ug. This data was developed using the same antibody clone with 13217-1-PBS in a different storage buffer formulation.

PNMT Polyclonal antibody, PBS Only

PNMT Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 13217-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

PNMTase, Phenylethanolamine N-methyltransferase, PENT, Noradrenaline N-methyltransferase, EC:2.1.1.28

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13217-1-PBS targets PNMT in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4098

Product name: Recombinant human PNMT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-282 aa of BC037246

Sequence: MSGADRSPNAGAAPDSAPGQAAVASAYQRFEPRAYLRNNYAPPRGDLCNPNGVGPWKLRCLAQTFATGEVSGRTLIDIGSGPTVYQLLSACSHFEDITMTDFLEVNRQELGRWLQEEPGAFNWSMYSQHACLIEGKGECWQDKERQLRARVKRVLPIDVHQPQPLGAGSPAPLPADALVSAFCLEAVSPDLASFQRALDHITTLLRPGGHLLLIGALEESWYLAGEARLTVVPVSEEEVREALVRSGYKVRDLRTYIMPAHLQTGVDDVKGVFFAWAQKVGL

相同性解析による交差性が予測される生物種
Full Name phenylethanolamine N-methyltransferase
Calculated molecular weight 282 aa, 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC037246
Gene Symbol PNMT
Gene ID (NCBI) 5409
RRIDAB_2167781
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP11086
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Phenylethanolamine N-methyltransferase(PNMT), the final enzyme in catecholamine biosynthesis, converts norepinephrine to epinephrine, thereby serving as a marker of the adrenergic phenotype.

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