Validation Data Gallery
Tested Applications
| Positive WB detected in | HT-1376 cells, TT cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21770-1-AP targets PNMA5 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16414 Product name: Recombinant human PNMA5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 133-448 aa of BC101112 Sequence: SLDAEVMPQVRSPPLEPPKESMWYRKLKVFSGTASPSPGEETFEDWLEQVTEIMPIWQVSEVEKRRRLLESLRGPALSIMRVLQANNDSITVEQCLDALKQIFGDKEDFGASQFRFLQTSPKIGEKVSTFLLRLEPLLQKAVHKSPLSVRSTDMIRLKHLLARVAMTPALRGKLELLDQRGCPPNFLELMKLIRDEEEWENTEAVMKNKEKPSGRGRGASGRQARAEASVSAPQATVQARSFSDSSPQTIQGGLPPLVKRRRLLGSESTRGEDHGQATYPKAENQTPGREGPQAAGEELGNEAGAGAMSHPKPWET 相同性解析による交差性が予測される生物種 |
| Full Name | paraneoplastic antigen like 5 |
| Calculated molecular weight | 448 aa, 50 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC101112 |
| Gene Symbol | PNMA5 |
| Gene ID (NCBI) | 114824 |
| RRID | AB_3669374 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96PV4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PNMA5 is a member of the paraneoplastic Ma antigen (PNMA) family proteins which share relatively conserved protein sequences and are involved in the regulation of growth, apoptosis, or fate determination in different cell types (PMID: 29378289). PNMA5 appears to be involved in the association areas of the neocortex during primate evolution (PMID: 19366867). PNMA5 has been reported to play roles in the pathological development and metastasis of some cancers, including colorectal cancer, ovarian cancer, and lung cancer (PMID: 35559417; 35117731; 34136487).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PNMA5 antibody 21770-1-AP | Download protocol |
| IHC protocol for PNMA5 antibody 21770-1-AP | Download protocol |
| WB protocol for PNMA5 antibody 21770-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |