Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 13631-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13631-1-AP (PNMA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 13631-1-AP
IP result of anti-PNMA1 (IP:13631-1-AP, 4ug; Detection:13631-1-AP 1:500) with mouse testis tissue lysate 4000ug.
IHC staining of mouse brain using 13631-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13631-1-AP (PNMA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 13631-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13631-1-AP (PNMA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 13631-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13631-1-AP (PNMA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 13631-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13631-1-AP (PNMA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 13631-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 13631-1-AP (PNMA1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human gliomas using 13631-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 13631-1-AP (PNMA1 antibody) at dilution of 1:100 (under 40x lens).

Featured Product
KD/KO Validated

PNMA1 Polyclonal antibody

PNMA1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 13631-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

37 kDa neuronal protein, MA1, paraneoplastic antigen MA1, PNMA1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	PC-3 cells, SH-SY5Y cells, mouse testis tissue, rat testis tissue
Positive IP detected in	mouse testis tissue
Positive IHC detected in	mouse brain tissue, mouse testis tissue, human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 4 publications below
IHC	See 1 publications below

Product Information

13631-1-AP targets PNMA1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4549 Product name: Recombinant human PNMA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-353 aa of BC039577 Sequence: MAMTLLEDWCRGMDVNSQRALLVWGIPVNCDEAEIEETLQAAMPQVSYRMLGRMFWREENAKAALLELTGAVDYAAIPREMPGKGGVWKVLFKPPTSDAEFLERLHLFLAREGWTVQDVARVLGFQNPTPTPGPEMPAEMLNYILDNVIQPLVESIWYKRLTLFSGRDIPGPGEETFDPWLEHTNEVLEEWQVSDVEKRRRLMESLRGPAADVIRILKSNNPAITTAECLKALEQVFGSVESSRDAQIKFLNTYQNPGEKLSAYVIRLEPLLQKVVEKGAIDKDNVNQARLEQVIAGANHSGAIRRQLWLTGAGEGPAPNLFQLLVQIREEEAKEEEEEAEATLLQLGLEGHF 相同性解析による交差性が予測される生物種
Full Name	paraneoplastic antigen MA1
Calculated molecular weight	353 aa, 40 kDa
Observed molecular weight	37 kDa
GenBank accession number	BC039577
Gene Symbol	PNMA1
Gene ID (NCBI)	9240
RRID	AB_2167615
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8ND90
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for PNMA1 antibody 13631-1-AP	Download protocol
IP protocol for PNMA1 antibody 13631-1-AP	Download protocol
WB protocol for PNMA1 antibody 13631-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Biomed Pharmacother PNMA1, regulated by miR-33a-5p, promotes proliferation and EMT in hepatocellular carcinoma by activating the Wnt/β-catenin pathway. Authors - Pengpeng Liu KD Validated View Article
human	WB,IHC	Int J Clin Exp Pathol PNMA1 promotes cell growth in human pancreatic ductal adenocarcinoma. Authors - Shu-Heng Jiang KD Validated View Article
human	WB	Res Sq The Retrotransposon-Derived Capsid Genes PNMA1 and PNMA4 Maintain Reproductive Capacity Authors - Luke Berchowitz View Article
mouse	WB	bioRxiv The retrotransposon - derived capsid genes PNMA1 and PNMA4 maintain reproductive capacity Authors - Thomas W P Wood KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 13631-1-AP

IP experiment of mouse testis using 13631-1-AP

IHC staining of mouse brain using 13631-1-AP

IHC staining of mouse brain using 13631-1-AP

IHC staining of mouse testis using 13631-1-AP

IHC staining of mouse testis using 13631-1-AP

IHC staining of human gliomas using 13631-1-AP