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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PNLDC1 Polyclonal antibody

PNLDC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25559-1-AP

Synonyms

PNLDC1

Validation Data Gallery

Applications:
  • WB analysis using 25559-1-AP

    WB analysis using 25559-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25559-1-AP (PNLDC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 25559-1-AP

    IHC staining of human testis using 25559-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25559-1-AP (PNLDC1 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human testis using 25559-1-AP

    IHC staining of human testis using 25559-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25559-1-AP (PNLDC1 Antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inRaji cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

25559-1-AP targets PNLDC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, silkworm
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PNLDC1 fusion protein Ag22074 相同性解析による交差性が予測される生物種
Full Name poly(A)-specific ribonuclease (PARN)-like domain containing 1
Calculated molecular weight 520 aa, 60 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC112246
Gene symbol PNLDC1
Gene ID (NCBI) 154197
RRIDAB_2880131
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PNLDC1, also named as Poly(A)-specific ribonuclease PARN-like domain-containing protein 1, is a 520 amino acid protein, which belongs to the CAF1 family. PNLDC1 as a membrane protein localizes in the membrane.

Protocols

Product Specific Protocols
WB protocol for PNLDC1 antibody 25559-1-APDownload protocol
IHC protocol for PNLDC1 antibody 25559-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
silkwormWB

Cell

Identification and Functional Analysis of the Pre-piRNA 3' Trimmer in Silkworms.

Authors - Natsuko Izumi
View Article
humanIHC

J Ovarian Res

A risk model of gene signatures for predicting platinum response and survival in ovarian cancer.

Authors - Siyu Chen
View Article