• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PNCK Polyclonal antibody

PNCK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13712-1-AP

Synonyms

BSTK3, CaM KI beta, CaM kinase I beta, CaM kinase IB, CaMK1b, CaMKI beta, PNCK

Validation Data Gallery

Applications:
  • WB analysis using 13712-1-AP

    WB analysis using 13712-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13712-1-AP (PNCK antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 13712-1-AP

    WB analysis of human brain using 13712-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13712-1-AP (PNCK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary using 13712-1-AP

    IHC staining of human ovary using 13712-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 13712-1-AP (PNCK antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary using 13712-1-AP

    IHC staining of human ovary using 13712-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 13712-1-AP (PNCK antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IHC detected inhuman ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:9000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13712-1-AP targets PNCK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PNCK fusion protein Ag4650 相同性解析による交差性が予測される生物種
Full Name pregnancy up-regulated non-ubiquitously expressed CaM kinase
Calculated molecular weight 47 kDa
Observed molecular weight 38 kDa, 45 kDa
GenBank accession numberBC033746
Gene symbol PNCK
Gene ID (NCBI) 139728
RRIDAB_10642952
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PNCK antibody 13712-1-APDownload protocol
IHC protocol for PNCK antibody 13712-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols