Validation Data Gallery
Tested Applications
| Positive WB detected in | LNCaP cells, rabbit brain tissue, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells |
| Positive IHC detected in | human tonsillitis tissue, Human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68905-5-Ig targets PMS2 in WB, IHC, ELISA applications and shows reactivity with human, rabbit samples.
| Tested Reactivity | human, rabbit |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PMS2 fusion protein Ag34237 相同性解析による交差性が予測される生物種 |
| Full Name | PMS2 postmeiotic segregation increased 2 (S. cerevisiae) |
| Calculated molecular weight | 862 aa, 96 kDa |
| Observed molecular weight | 117 kDa, 100 kDa |
| GenBank accession number | BC093921 |
| Gene Symbol | PMS2 |
| Gene ID (NCBI) | 5395 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P54278 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PMS2, also named as PMSL2, belongs to the DNA mismatch repair mutL/hexB family. It is a component of the post-replicative DNA mismatch repair system (MMR). It heterodimerizes with MLH1 to form MutL alpha. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. It also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. (PMID: 16873062, PMID: 18206974) Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4). Defects in PMS2 are a cause of mismatch repair cancer syndrome (MMRCS).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PMS2 antibody 68905-5-Ig | Download protocol |
| IHC protocol for PMS2 antibody 68905-5-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |