Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 2 publications below |
Product Information
10859-1-AP targets PMS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PMS1 fusion protein Ag1158 相同性解析による交差性が予測される生物種 |
| Full Name | PMS1 postmeiotic segregation increased 1 (S. cerevisiae) |
| Calculated molecular weight | 106 kDa |
| GenBank accession number | BC008410 |
| Gene Symbol | PMS1 |
| Gene ID (NCBI) | 5378 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P54277 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The multiple eukaryotic homologs of the bacterial MutL mismatch repair protein are implicated along with MutS homologs in maintaining genomic integrity during DNA replication and recombination [PMID:16612326]. DNA mismatch repair (MMR) corrects replication errors that would otherwise lead to mutations and, potentially, various forms of cancer. Among several proteins required for eukaryotic MMR, MutLα is a heterodimer comprised of Mlh1 and PMS1 [PMID:19115045]. PMS1 contains an N-terminal domain (NTD) and C-terminal domain (CTD) ,which separated by a flexible linker. Dimerization occurs between the CTDs and the CTD of PMS1 houses a strand-specific endonuclease that is necessary for MMR [PMID:17951253].
Publications
| Species | Application | Title |
|---|---|---|
Breast Cancer Res Tumor sequencing is useful to refine the analysis of germline variants in unexplained high-risk breast cancer families. | ||