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Validation Data Gallery

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  • Western Blot (WB) analysis of multi-tissue using PMM2 Polyclonal antibody (10666-1-AP)

    WB analysis of multi-tissue using 10666-1-AP (same clone as 10666-1-PBS)

    WB results of 10666-1-AP (PMM2 antibody) on several lysates. This data was developed using the same antibody clone with 10666-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PMM2 Polyclonal antibody (10666-1-AP)

    WB analysis of HepG2 using 10666-1-AP (same clone as 10666-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10666-1-AP (PMM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10666-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PMM2 Polyclonal antibody (10666-1-AP)

    WB analysis of HeLa using 10666-1-AP (same clone as 10666-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10666-1-AP (PMM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10666-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using PMM2 Polyclonal antibody (10666-1-AP)

    IP experiment of mouse lung using 10666-1-AP (same clone as 10666-1-PBS)

    IP result of anti-PMM2 (IP:10666-1-AP, 4ug; Detection:10666-1-AP 1:1000) with mouse lung tissue lysate 3600ug. This data was developed using the same antibody clone with 10666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PMM2 Polyclonal antibody (10666-1-AP)

    IHC staining of human breast cancer using 10666-1-AP (same clone as 10666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10666-1-AP (PMM2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PMM2 Polyclonal antibody (10666-1-AP)

    IHC staining of human breast cancer using 10666-1-AP (same clone as 10666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10666-1-AP (PMM2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PMM2 Polyclonal antibody (10666-1-AP)

    IHC staining of human breast cancer using 10666-1-AP (same clone as 10666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10666-1-AP (PMM2 antibody) at dilution of 1:100 (under 25x lens). This data was developed using the same antibody clone with 10666-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PMM2 Polyclonal antibody (10666-1-AP)

    IF Staining of HepG2 using 10666-1-AP (same clone as 10666-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PMM2 antibody (10666-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10666-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PMM2 Polyclonal antibody, PBS Only

PMM2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10666-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

phosphomannomutase 2, EC:5.4.2.8

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10666-1-PBS targets PMM2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1031

Product name: Recombinant human PMM2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-246 aa of BC008310

Sequence: MAAPGPALCLFDVDGTLTAPRQKITKEMDDFLQKLRQKIKIGVVGGSDFEKVQEQLGNDVVEKYDYVFPENGLVAYKDGKLLCRQNIQSHLGEALIQDLINYCLSYIAKIKLPKKRGTFIEFRNGMLNVSPIGRSCSQEERIEFYELDKKENIRQKFVADLRKEFAGKGLTFSIGGQISFDVFPDGWDKRYCLRHVENDGYKTIYFFGDKTMPGGNDHEIFTDPRTMGYSVTAPEDTRRICELLFS

相同性解析による交差性が予測される生物種
Full Name phosphomannomutase 2
Calculated molecular weight 28 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC008310
Gene Symbol PMM2
Gene ID (NCBI) 5373
RRIDAB_2166973
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO15305
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PMM2(Phosphomannomutase 2) belongs to the eukaryotic PMM family. It is involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. PMM2 catalyzes the second step in the conversion of fructose-6P to GDP-mannose.

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