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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PMF1 Polyclonal antibody

PMF1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11190-2-AP

Synonyms

PMF 1, PMF1, polyamine modulated factor 1

Validation Data Gallery

Applications:
  • WB analysis of Jurkat using 11190-2-AP

    WB analysis of Jurkat using 11190-2-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11190-2-AP (PMF1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 11190-2-AP

    WB analysis of HepG2 using 11190-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11190-2-AP (PMF1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 11190-2-AP

    IHC staining of human gliomas using 11190-2-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11190-2-AP (PMF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • PMF1 Antibody IHC human gliomas tissue 11190-2-AP

    IHC staining of human gliomas tissue using 11190-2-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11190-2-AP( PMF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 11190-2-AP

    IF Staining of HepG2 using 11190-2-AP

    Immunofluorescent analysis of HepG2 cells using 11190-2-AP (PMF1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, HepG2 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11190-2-AP targets PMF1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PMF1 fusion protein Ag1672 相同性解析による交差性が予測される生物種
Full Name polyamine-modulated factor 1
Calculated molecular weight 19 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC033656
Gene symbol PMF1
Gene ID (NCBI) 11243
RRIDAB_2064906
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PMF1 antibody 11190-2-APDownload protocol
IHC protocol for PMF1 antibody 11190-2-APDownload protocol
IF protocol for PMF1 antibody 11190-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols