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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PMCA2 Polyclonal antibody (28423-1-AP)

    WB analysis using 28423-1-AP (same clone as 28423-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28423-1-AP (PMCA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28423-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PMCA2 Polyclonal antibody (28423-1-AP)

    IHC staining of mouse brain using 28423-1-AP (same clone as 28423-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28423-1-AP (PMCA2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28423-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using PMCA2 Polyclonal antibody (28423-1-AP)

    IHC staining of rat brain using 28423-1-AP (same clone as 28423-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28423-1-AP (PMCA2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28423-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using PMCA2 Polyclonal antibody (28423-1-AP)

    IF Staining of rat brain using 28423-1-AP (same clone as 28423-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using PMCA2 antibody (28423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28423-1-PBS in a different storage buffer formulation.

PMCA2 Polyclonal antibody, PBS Only

PMCA2 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 28423-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28423-1-PBS targets PMCA2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag28994

Product name: Recombinant human PMCA2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 578-782 aa of NM_001001331

Sequence: LDLKQDYEPVRSQMPEEKLYKVYTFNSVRKSMSTVIKLPDESFRMYSKGASEIVLKKCCKILNGAGEPRVFRPRDRDEMVKKVIEPMACDGLRTICVAYRDFPSSPEPDWDNENDILNELTCICVVGIEDPVRPEVPEAIRKCQRAGITVRMVTGDNINTARAIAIKCGIIHPGEDFLCLEGKEFNRRIRNEKGEIEQERIDKIW

相同性解析による交差性が予測される生物種
Full Name ATPase, Ca++ transporting, plasma membrane 2
Calculated molecular weight 137 kDa
Observed molecular weight 137 kDa
GenBank accession numberNM_001001331
Gene Symbol PMCA2
Gene ID (NCBI) 491
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01814
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ATP2B2, also named as PMCA2, belongs to the cation transport ATPase (P-type) family and type IIB subfamily. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

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