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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PM20D1 Recombinant antibody (84397-2-RR)

    WB analysis using 84397-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84397-2-RR (PM20D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84397-2-RR

    Affinity and Kinetic Characterization of 84397-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84397-2-RR against Human PM20D1 were performed. The affinity constant is 0.126 nM.

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PM20D1 Recombinant antibody

PM20D1 Recombinant Antibody for WB, ELISA
Cat No. 84397-2-RR
Clone No.241557E1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Peptidase M20 domain-containing protein 1, N-fatty-acyl-amino acid synthase/hydrolase PM20D1, EC:3.5.1.114, 241557E1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse kidney tissue, mouse pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84397-2-RR targets PM20D1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PM20D1 fusion protein Ag29154 相同性解析による交差性が予測される生物種
Full Name peptidase M20 domain containing 1
Observed molecular weight 55-60 kDa
GenBank accession numberBC063477
Gene Symbol PM20D1
Gene ID (NCBI) 148811
RRIDAB_3671928
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6GTS8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PM20D1 is a secreted enzyme that regulates the endogenous N-fatty acyl amino acid (NAAs) tissue and circulating levels by functioning as a bidirectional NAA synthase/hydrolase (PMID: 27374330).

Protocols

Product Specific Protocols
WB protocol for PM20D1 antibody 84397-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols